The g.tec 16-channel head-stages allow the recording of action potentials/spikes with g.HIamp or g.USBamp. The boards are extremely small and lightweight and are connected via a very flexible cable to the amplifier. This allows the animal to move with almost no restrictions. The electronic system produces recordings with very little noise and very high input impedance, thereby providing top quality data. The channels have very similar gain, and thus a very high Common Mode Rejection Ratio is obtained. The head-stages can also be used for biosignal recordings like EEG or ECG in animals.
g.HEADstages are available with system connectors or with single channel connectors that can be interfaced to electrodes/wires easily. The head-stages are connected to the g.SPIKEsens power supply and filtering box that is connected to the amplifier. One g.SPIKEsens box supports up to 16 channels. Several head-stages can be used simultaneously. The g.SPIKEsens box filters the signal between 0.5 Hz and 6000 Hz. Voltage Noise Density at 1 kHz is only 10 nV/SQRT(Hz). Aggregation boards can be used to interface the head-stages with different electrodes like twisted wires, silicon electrodes and/or multi-electrode arrays.
Importantly, the flexible cable can be disconnected from the head-stage. This allows you to replace the cable if it is destroyed by an animal or any other cause.
The g.HEADstage is offered in two different types:
- g.HEADstage, 16 channels with multi-pin connector
- g.HEADstage, 16 channels with golden-pin connector
|Extremely small board|
|Aggregation boards help interface different electrodes types|
|High quality neuronal activity recordings|
|LED slot to support easier video tracking|
|Very high signal-to-noise ratio|
|Size||23 x 23 mm|
|Input channels||16 monopolar|
|Filter||0.5 – 6000 Hz|
|Safety class||Internally powered|
RECORDING OF PLACE CELLS IN RATS
In-vivo recordings of action potentials (spikes) in rats can be done by using the g.HEADstages, the biosignal amplifier g.USBamp and the real-time processing software g.USBamp SIMULINK HIGH-SPEED ONLINE Processing. An example is the real-time reconstructions of movement trajectories of rats while they are foraging for food in an arena. The rat’s path is investigated with a video camera-based tracking system that transmits the x- and y- positions to a recording computer. Simultaneously, the neuronal activity is recorded with tetrodes (electrodes consisting of 4 wires) placed in the CA1 region of the hippocampus. This is a typical setup for place and grid cell recordings. These neurons increase their firing activity only when the animal is at a specific location in its environment and a field where a neuron fires is called place field. By recording from many of these place and grid cells, the rat’s position in the arena can be decoded using BCI technology.